FastQCFastQC Report
Sun 19 Mar 2023
SRR4895921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329498
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG50340.37863915553088456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC37490.28198613311189635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC35360.26596504846190067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34900.26250509590838045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG27730.2085749658893808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC18660.14035372749714553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT17070.12839432627954311No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC16310.12267788293024887No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC15690.1180144686189825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC15220.11447929970560317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG14850.11169629439081517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303750.0138.900948
GTGGTAT305750.0137.945256
TGGTATC307600.0137.185847
AAGCAGT308400.0136.731031
GTATCAA310250.0136.037269
AGTGGTA310100.0136.010215
CAGTGGT313050.0134.728524
AGCAGTG313100.0134.609532
GCAGTGG315550.0133.610053
CAACGCA301900.028.53232410-14
TCAACGC302600.028.46156510-14
ATCAACG304800.028.24195910-14
GTACATG302250.028.22772820-24
TACATGG302900.028.14338320-24
AGAGTAC305450.028.0498615-19
AACGCAG307800.027.95266210-14
AGTACAT305800.027.92828820-24
CGCAGAG307650.027.92416615-19
CAGAGTA307850.027.86392615-19
GAGTACA304150.027.5542820-24
Produced by FastQC (version 0.11.5)