FastQCFastQC Report
Sun 19 Mar 2023
SRR4895920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806546
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA810600.0141.817292
TCTTCAC811700.0141.62511
TTCACAG255550.0140.864943
TCACATG79200.0140.648534
TTCACAT262750.0140.097063
TCACAGT83200.0139.45454
TCACAGA62300.0138.191184
TCACAGG85200.0137.85394
TCACATA70200.0137.563634
TCACAAT70950.0137.314854
TTCACAA206650.0136.364933
TCACATT84250.0136.278434
TCACAAG66500.0135.679264
TCACACG26250.0134.936224
TTCACAC103750.0134.638533
CACAGAT21750.0134.018725
CACATAG23750.0133.535845
TCACATC34100.0132.924224
CACATGG28450.0131.766465
TCACAGC30750.0131.413314
Produced by FastQC (version 0.11.5)