FastQCFastQC Report
Sun 19 Mar 2023
SRR4895920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806546
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG179682.2277712616515366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC116011.4383556548541558No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33710.41795508253713987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC20940.2596256134182055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC15640.19391330438685456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA14790.18337453784409072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAAAAAAAAAAAAAA14690.18213468295670676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA11810.1464268622000481No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA11570.14345121047032655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT10890.13502019723611547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAGCCCTGGACTCATCAT9500.11778621430147815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG9470.11741425783526296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCACAAAAAAAAAAAAA9190.11394266415058782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT479300.0141.848391
GTATCAA486400.0140.131629
GGTATCA486600.0140.118428
GTGGTAT488050.0139.598866
CAGTGGT490300.0139.031654
TGGTATC490550.0138.990167
AGCAGTG489700.0138.86532
AGTGGTA494000.0137.975755
GCAGTGG494800.0137.608693
CGCACGA255.3688535E-428.8007860-64
CAACGCA486050.028.62420310-14
ATCAACG485600.028.61514510-14
TCAACGC486450.028.59770610-14
AGAGTAC484500.028.57906315-19
GTACATG483250.028.5427420-24
AGTACAT483950.028.51038220-24
CGCAGAG487350.028.5035315-19
CAGAGTA486300.028.4999315-19
TACATGG484050.028.45392220-24
GCAGAGT487150.028.42655615-19
Produced by FastQC (version 0.11.5)