FastQCFastQC Report
Sun 19 Mar 2023
SRR4895919_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895919_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590420
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA1592800.0142.335332
CGTCTAA394900.0141.972553
CGTCTAG503850.0141.970143
CGTCTAT487050.0141.891593
TCCGTCT1598600.0141.818911
CGTCTAC212700.0141.075683
GTCTAAG133300.0140.411334
GTCTAGG166400.0140.237094
GTCTATG160000.0140.189134
GTCTAGT165300.0140.00614
GTCTATA127400.0139.24934
GTCTAGA125350.0139.138464
GTCTAAT131900.0139.03774
GTCTACG56500.0138.76564
GTCTACC72150.0137.709534
GTCTAGC55350.0137.528034
GTCTATC65000.0137.396384
TCTAGTG57550.0136.109955
TCTAGTA45600.0135.98535
GTCTAAC34250.0135.890844
Produced by FastQC (version 0.11.5)