FastQCFastQC Report
Sun 19 Mar 2023
SRR4895919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590420
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG185261.164849536600395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC115260.7247142264307542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC108620.6829642484375197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG66150.4159278681103105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33110.2081840017102401No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCTTGCTGCAGCAACG25650.16127815294073264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC22750.14304397580513323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC19680.12374089863055042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC19540.12286062801021114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC17870.11236025703902114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT17030.10707863331698546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA402750.0138.86378
AAGCAGT402500.0138.769331
GTGGTAT405150.0137.86346
TGGTATC407550.0137.122217
GTATCAA409800.0136.457189
AGTGGTA411150.0135.869035
AGCAGTG412350.0135.402072
CAGTGGT412800.0135.360844
GCAGTGG418650.0133.41613
TCAACGC401500.028.47642310-14
CAACGCA401900.028.4552510-14
ATCAACG403600.028.29257810-14
GTACATG402050.028.26161820-24
TACATGG401550.028.24301720-24
AGAGTAC404650.028.1627615-19
CAGAGTA406350.028.05202515-19
AGTACAT405100.028.04883620-24
CGCAGAG408200.027.93900115-19
AACGCAG411850.027.77128810-14
GAGTACA402150.027.67095220-24
Produced by FastQC (version 0.11.5)