FastQCFastQC Report
Sun 19 Mar 2023
SRR4895918_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895918_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826818
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC1829100.0142.373211
ATCAGCA1829600.0142.33432
TCAGCAG591400.0141.841383
TCAGCAT552700.0141.688433
TCAGCAA449250.0140.775533
TCAGCAC249050.0140.434653
CAGCATG183500.0139.095474
CAGCAGT188950.0138.893424
CAGCAAT149000.0138.247184
CAGCATA145550.0137.70444
CAGCATT167300.0137.35484
CAGCAGG208200.0135.979454
CAGCAAG152350.0135.675144
CAGCAGA149200.0135.482164
AGCAGTG65850.0133.676775
CAGCACG70200.0133.414494
AGCATGT61150.0133.344255
CAGCATC73650.0132.584434
AGCATTG60000.0132.33625
AGCATGG69000.0132.325885
Produced by FastQC (version 0.11.5)