FastQCFastQC Report
Sun 19 Mar 2023
SRR4895918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826818
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG295531.6177309398090014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC198551.086862511755413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC128630.7041204980463297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG79800.43682512434188847No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40320.22071164177274363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC32170.17609854949973122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA28740.1573227327517027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC25120.13750685618381253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCTTGCTGCAGCAACG24610.1347151166673418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC21780.11922369935045526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG19930.10909680110443405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA456000.0138.53568
AAGCAGT456500.0138.2521
GTGGTAT457600.0138.035486
TGGTATC459250.0137.570897
GTATCAA460650.0137.215329
CAGTGGT466050.0135.625444
AGCAGTG466400.0135.178422
AGTGGTA470550.0134.29785
GCAGTGG473500.0133.455673
TCAACGC454300.028.47643710-14
CAACGCA455350.028.4234210-14
ATCAACG456550.028.30770910-14
GTACATG453950.028.27982320-24
CAGAGTA456400.028.26022115-19
AGAGTAC457350.028.21726215-19
TACATGG454650.028.18560420-24
AGTACAT457450.028.07604220-24
CGCAGAG462300.027.95250915-19
GAGTACA455000.027.62273620-24
GCAGAGT466900.027.59075715-19
Produced by FastQC (version 0.11.5)