FastQCFastQC Report
Sun 19 Mar 2023
SRR4895917_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895917_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641759
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG643450.0142.165441
AGGATGA644500.0141.933822
GGATGAC87300.0141.399083
GGATGAT216700.0141.160683
GGATGAA161950.0140.69373
GGATGAG183150.0140.635243
GATGACC29550.0138.441834
GATGACT22150.0138.037324
GATGATA60500.0137.12314
GATGACG21950.0136.372794
GATGATC25800.0136.188434
GATGAAT55750.0135.255144
GATGATG71550.0135.071274
GATGAGT59600.0134.64964
GATGAAG50800.0134.26454
GATGAGC26150.0133.820534
GATGAGG59850.0133.372674
GATGAGA47200.0132.425174
GATGATT68950.0132.205414
GATGACA16750.0131.479064
Produced by FastQC (version 0.11.5)