FastQCFastQC Report
Sun 19 Mar 2023
SRR4895917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641759
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA110221.717467148882992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA44670.6960556844547563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG41540.6472834818054752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28400.4425337237187168No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20950.32644653210940555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC20270.31585065421754893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC15510.24167950897455276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA12960.20194496688009048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG12320.191972375923049No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA11600.1807532110963773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC11200.17452034174822637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT10850.1690665810685943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC9190.14320017327376788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT7080.11032178746227166No Hit
AAGCAGTGGTATCAACGCAGAGAACATGGGGAAAAAAAAAAAAAAAAAAA6590.10268652251078675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT157850.0139.03881
GGTATCA158000.0139.032688
GTGGTAT158750.0138.285136
GTATCAA159050.0138.02439
TGGTATC159950.0137.337697
AGCAGTG161450.0136.027712
CAGTGGT162250.0135.479584
AGTGGTA162250.0135.346475
GCAGTGG164250.0133.708833
AGAGTAC155800.028.31009515-19
CAACGCA157900.028.3074910-14
GTACATG155700.028.15255520-24
TCAACGC158850.028.14726310-14
ATCAACG158750.028.1287110-14
TACATGG155700.028.07856820-24
CGCAGAG159250.028.04039415-19
CAGAGTA157250.028.02157815-19
AGTACAT156750.027.945620-24
GCAGAGT158600.027.7739815-19
GAGTACA155550.027.70757320-24
Produced by FastQC (version 0.11.5)