FastQCFastQC Report
Sun 19 Mar 2023
SRR4895916_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895916_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577989
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCTA581550.0141.658252
GTTCTAG184500.0141.30453
GTTCTAA137600.0141.142143
GTTCTAT193800.0140.554763
GTGTTCT587050.0140.331051
GTTCTAC69100.0139.652663
TTCTAGG61250.0138.002614
TCTAGAG15600.0137.515155
TTCTATG68350.0137.431264
TTCTAGA46050.0136.9694
TCTATGA15950.0136.284935
TCTAGAT14650.0136.21635
TCTATAG16250.0135.961825
TCTAATG15050.0135.910815
TCTATGG24200.0135.177985
TTCTAGT62850.0134.262594
TCTAAGG18400.0134.019175
TTCTACG19150.0133.608584
TCTAGTA14850.0133.421865
TCTAGTG21550.0132.618995
Produced by FastQC (version 0.11.5)