FastQCFastQC Report
Sun 19 Mar 2023
SRR4895915_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895915_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998383
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA2005400.0142.046052
GTCTGTC2005900.0142.010651
CTGTCAG636850.0141.685423
CTGTCAT615150.0141.307433
CTGTCAA502400.0141.000843
CTGTCAC264700.0139.262183
TGTCAGG219900.0139.111534
TGTCATG205000.0138.479374
TGTCAAG163300.0138.357184
TGTCAGT197150.0138.31744
TGTCAGA155650.0138.151254
TGTCAAT167650.0137.785694
TGTCATA166700.0137.758544
GTCATAG57200.0134.946325
GTCAGAT51700.0134.827035
TGTCACG71250.0134.344454
GTCATGA50950.0134.293725
TGTCAGC80950.0134.270814
GTCAATG58300.0133.989475
TGTCATT181000.0133.293434
Produced by FastQC (version 0.11.5)