FastQCFastQC Report
Sun 19 Mar 2023
SRR4895915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998383
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG137240.686755241612844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT100120.5010050625931065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC94380.4722818398675329No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52280.2616115129081863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC49130.24584876872951783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC48860.24449767637134626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA41540.20786806132758337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCCCCTCATTTACATAAA39020.1952578659846486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA34710.17369042871161333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC27440.13731101595640074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC23160.11589370005649569No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC22550.1128412321361821No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT22540.11279119167847206No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG20940.10478471844486267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA539200.0138.350258
AAGCAGT541350.0137.726361
GTGGTAT544400.0137.068426
GTATCAA545750.0136.689779
TGGTATC546800.0136.453637
AGTGGTA548400.0136.081775
CAGTGGT554400.0134.72594
AGCAGTG553400.0134.688392
GCAGTGG556250.0134.166643
CAACGCA534450.028.50602710-14
TCAACGC535450.028.44472310-14
ATCAACG538550.028.23018610-14
GTACATG535400.028.2190520-24
TACATGG536700.028.10508320-24
AGAGTAC541200.028.07865115-19
AACGCAG543400.028.05242310-14
CGCAGAG542200.028.04811115-19
CAGAGTA543850.027.9471315-19
AGTACAT542900.027.84777820-24
GCAGAGT549150.027.64593915-19
Produced by FastQC (version 0.11.5)