FastQCFastQC Report
Sun 19 Mar 2023
SRR4895914_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895914_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617547
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG618200.0142.38751
TCGTAGA618200.0142.38752
CGTAGAG197000.0142.006213
CGTAGAT195800.0141.857273
CGTAGAA149400.0141.83333
CGTAGAC78500.0141.186983
GTAGATA52700.0139.57354
GTAGATG68250.0138.476074
GTAGAGT59350.0138.465774
GTAGACT18700.0138.35634
GTAGAGG69950.0137.96374
GTAGACC27750.0137.4124
GTAGAGA47850.0137.186574
GTAGAGC25900.0136.76994
GTAGAAT53900.0136.730594
GTAGAAG51400.0136.44764
GTAGACG21000.0136.439674
TAGATAG17950.0135.798685
GTAGATT53350.0133.731454
TAGAATG19350.0132.235295
Produced by FastQC (version 0.11.5)