FastQCFastQC Report
Sun 19 Mar 2023
SRR4895914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617547
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG77661.2575561050413975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC59190.9584695577826464No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC31000.5019860836503133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC21050.3408647438980353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG14010.22686532361099643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT13740.2224931867533969No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA13670.22135966979031554No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13370.2165017399485383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT13150.21293925806456837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC7870.12743969284928922No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA7710.12484879693367469No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG7370.11934314311299384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA6970.11286590332395753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA6680.10816990447690622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC6640.10752218049800259No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA6610.10703638751382485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT187450.0139.001311
GGTATCA187800.0138.823588
GTGGTAT189050.0137.905696
GTATCAA189850.0137.248729
TGGTATC190150.0137.145777
CAGTGGT190400.0137.116944
AGCAGTG191850.0135.813392
AGTGGTA192800.0135.223375
GCAGTGG193850.0134.560763
CAACGCA186800.028.5223410-14
AGAGTAC187250.028.37689415-19
TACATGG186750.028.34491720-24
GTACATG187400.028.30038820-24
ATCAACG187850.028.27859110-14
TCAACGC188650.028.27316710-14
CAGAGTA188050.028.26382815-19
AGTACAT187900.028.17143820-24
CGCAGAG189100.028.11450815-19
GCAGAGT190200.027.92919715-19
GAGTACA186850.027.7825720-24
Produced by FastQC (version 0.11.5)