FastQCFastQC Report
Sun 19 Mar 2023
SRR4895913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007038
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA1008050.0142.402951
TACGCAA1008300.0142.367652
ACGCAAT319300.0142.082673
CGCAATG112050.0141.470064
ACGCAAG335000.0141.189763
CGCAATA77650.0140.89924
CGCAAGT102000.0140.594854
ACGCAAA223450.0139.99963
CGCAATT87900.0139.145774
CGCAAGA79750.0138.976494
ACGCAAC141100.0138.914413
CGCAAGG119200.0138.724564
CGCAAAG84950.0137.936774
CGCAAAT74800.0137.882314
CGCAATC46500.0136.42024
CGCAACT31000.0135.65384
GCAATCG15800.0135.333655
GCAATGT37650.0135.168295
GCAAGTG37800.0133.68915
GCAATAG29150.0132.770895
Produced by FastQC (version 0.11.5)