FastQCFastQC Report
Sun 19 Mar 2023
SRR4895913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007038
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG54430.5404959892278146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43050.4274913161171674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC33920.33682939471996093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC26700.26513398699949753No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22600.22442052832167209No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG16590.16474055596710352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT13710.1361418337738993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC10940.1086354238866855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC10430.10357106683163893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA240050.0137.996228
AAGCAGT241800.0136.946371
GTGGTAT243000.0136.143166
GTATCAA244000.0135.762259
TGGTATC244750.0135.346247
AGTGGTA244700.0135.226765
CAGTGGT246350.0134.408724
AGCAGTG246450.0134.391712
GCAGTGG251550.0131.523833
CAACGCA239350.028.35977610-14
TCAACGC239750.028.30645210-14
ATCAACG240500.028.18225510-14
AGAGTAC240600.028.08675215-19
GTACATG240300.028.05590220-24
TACATGG240800.028.00362820-24
CAGAGTA242100.027.93057615-19
CGCAGAG242650.027.89100515-19
AGTACAT242150.027.79398520-24
AACGCAG244700.027.73972910-14
GAGTACA240000.027.47299620-24
Produced by FastQC (version 0.11.5)