FastQCFastQC Report
Sun 19 Mar 2023
SRR4895912_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895912_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862146
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA864300.0142.181952
GGTGCGA865050.0142.058671
TGCGAAG278600.0141.623813
TGCGAAT279450.0141.60113
GCGAATG93550.0141.021064
TGCGAAC112300.0140.767733
GCGAAGT87200.0140.583154
TGCGAAA199750.0140.43963
GCGAAAT69450.0139.979164
GCGAATA71700.0139.761474
GCGAATT73750.0139.645554
CGAAGTA24800.0138.808735
GCGAATC43650.0138.789294
CGAATAG25600.0138.368655
GCGAACT25100.0138.285464
GCGAAGA67950.0137.719854
CGAATTG23350.0137.661015
CGAATGA22350.0137.123615
CGAATGT31950.0136.967915
GCGAAGG94850.0136.758834
Produced by FastQC (version 0.11.5)