FastQCFastQC Report
Sun 19 Mar 2023
SRR4895912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862146
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG83450.967933505461952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC67010.7772465452487166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC34600.4013241376750573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC29670.34414124753812003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG25730.2984413312826366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21590.25042162232382914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC21380.24798583998533894No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT16350.18964305349673954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC15230.17665221435812495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA14890.1727085667624741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT14660.17004080515365147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA14470.16783700208549365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG12460.1445230854170871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT12160.1410433963621011No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG11340.13153224627847254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACTCTTAGCGGTGGATCA9410.10914624669139565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237000.0139.225628
GTGGTAT237250.0138.836126
AAGCAGT238900.0138.045841
GTATCAA240150.0137.42949
TGGTATC240250.0137.252327
CAGTGGT243050.0135.641514
AGTGGTA243200.0135.469045
AGCAGTG243700.0135.208622
GCAGTGG247950.0132.920123
CAACGCA235550.028.5972810-14
TCAACGC237200.028.39228210-14
ATCAACG237450.028.35026210-14
GTACATG236650.028.30615220-24
TACATGG236600.028.26953320-24
AGTACAT238150.028.13995720-24
CAGAGTA239150.028.08852215-19
CGCAGAG239800.028.06042515-19
AGAGTAC239850.028.01855315-19
GCAGAGT241250.027.83805315-19
GAGTACA237400.027.71935720-24
Produced by FastQC (version 0.11.5)