FastQCFastQC Report
Sun 19 Mar 2023
SRR4895911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962220
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG93370.9703602086840848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC69170.7188584731142567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC36430.3786036457359024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG25410.2640768223483195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC23990.2493192824925693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19410.2017210201409241No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC18670.194030471202012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA14420.1498617779717736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT13990.1453929454802436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA10500.10912265386294195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT247500.0139.42321
GGTATCA248050.0139.382648
GTGGTAT248700.0138.75786
TGGTATC251200.0137.577487
GTATCAA252000.0137.312169
CAGTGGT254900.0135.523994
AGTGGTA255550.0135.122925
AGCAGTG255500.0134.91682
GCAGTGG257150.0134.13513
CAACGCA247600.028.51458210-14
TCAACGC248100.028.48033110-14
GTACATG246400.028.4033420-24
AGAGTAC247450.028.39802415-19
ATCAACG248900.028.3425110-14
CAGAGTA248250.028.28330615-19
TACATGG247650.028.20182420-24
TACGCAC4700.028.18679650-54
AGTACAT248450.028.14579220-24
CGCAGAG251200.028.01421515-19
GCAGAGT253350.027.69122115-19
Produced by FastQC (version 0.11.5)