FastQCFastQC Report
Sun 19 Mar 2023
SRR4895910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804702
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA805750.0142.360412
GCTCGGT806900.0142.157521
TCGGTAG269150.0141.990083
TCGGTAA186950.0141.859923
TCGGTAT239050.0141.741153
TCGGTAC113750.0141.418333
CGGTAGT83200.0141.175544
CGGTATG85900.0140.720784
CGGTATA59400.0140.626364
CGGTAGA64250.0140.105684
CGGTAGG95250.0139.777544
CGGTAAG64650.0139.349084
CGGTAAT59000.0139.284534
CGGTATT66000.0138.658544
CGGTACG29950.0137.548724
CGGTAGC30250.0137.362674
CGGTATC31300.0137.081154
CGGTAAA48250.0136.046664
CGGTACC39750.0135.015434
CGGTACA22600.0132.769784
Produced by FastQC (version 0.11.5)