FastQCFastQC Report
Sun 19 Mar 2023
SRR4895909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875689
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA876550.0142.398322
GCTAACG876650.0142.382081
TAACGAG289450.0142.24923
TAACGAT266500.0141.902833
TAACGAC117550.0141.756473
TAACGAA206000.0141.575933
AACGAGT81900.0141.065284
AACGATG91250.0140.904454
AACGATC30050.0139.935714
AACGATA70600.0139.415164
ACGATAG22400.0139.04475
AACGAAG70500.0138.601964
AACGAAT71200.0138.540624
AACGAGG103700.0138.420914
AACGACT27500.0138.397924
ACGATGA23600.0138.014665
ACGAGTG27700.0137.913275
AACGATT79300.0137.601464
ACGATTG28000.0137.199255
ACGATGT29200.0136.686685
Produced by FastQC (version 0.11.5)