FastQCFastQC Report
Sun 19 Mar 2023
SRR4895909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875689
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG79260.9051158573420472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC53000.6052377042534507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC33620.3839262569245474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC31900.3642845804846241No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG21490.24540675970578596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20930.23901179528348532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA17390.19858648447108507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15350.17529054264698996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT13980.15964571897100455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA9770.1115692900104946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT9630.10997054890491945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT8800.10049229806472389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222150.0139.331568
AAGCAGT223450.0138.334881
GTGGTAT224550.0137.682056
TGGTATC225350.0137.225227
GTATCAA226350.0136.905269
CAGTGGT226600.0136.53184
AGCAGTG227700.0135.563112
AGTGGTA228600.0135.30585
GCAGTGG232700.0132.928823
CAACGCA221450.028.52661710-14
ATCAACG222350.028.37229510-14
TCAACGC223150.028.2899410-14
GTACATG221900.028.2546220-24
AGAGTAC223450.028.18751315-19
AGTACAT222850.028.14063320-24
TACATGG222500.028.11370820-24
CGCAGAG224200.028.10606815-19
CAGAGTA224600.028.06242215-19
GAGTACA222450.027.72515720-24
AACGCAG228700.027.60970710-14
Produced by FastQC (version 0.11.5)