FastQCFastQC Report
Sun 19 Mar 2023
SRR4895908_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895908_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758198
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA1759800.0142.418751
CGAGTAA1760000.0142.402562
GAGTAAG603050.0141.725043
GAGTAAT555150.0141.3073
GAGTAAC228400.0140.617583
GAGTAAA392300.0139.1733
AGTAACG64200.0138.55564
AGTAAGG221250.0138.042274
AGTAATG193050.0137.641894
AGTAATC79650.0137.630634
AGTAAGT198050.0136.938114
AGTAAGA139100.0136.762184
AGTAACC81250.0136.674854
AGTAATA144550.0136.092914
AGTAAGC64800.0135.512774
AGTAACT52650.0133.48214
GTAATAG54850.0133.066245
AGTAATT159650.0133.042954
GTAACCG20000.0132.217355
AGTAAAG155500.0131.918264
Produced by FastQC (version 0.11.5)