FastQCFastQC Report
Sun 19 Mar 2023
SRR4895908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758198
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG243471.3847700884655767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC163860.9319769445762082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64010.3640659356909745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC35260.20054624109457522No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATGCAAATGTTTTGAAA32930.18729403628032792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA32620.1855308673994624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC31420.17870569753804746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG26010.1479355567461685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT25030.142361668026013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA22270.12666377734475867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC19720.11216029138925196No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC19500.11090901024799255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCATTTGCACTGGTTGG18600.10579013285193135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC18550.1055057507743724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT17860.10158127810405881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT789400.0140.996921
GGTATCA799350.0139.348548
GTGGTAT798900.0139.32796
GTATCAA803200.0138.743339
TGGTATC803600.0138.584697
AGCAGTG804350.0138.250922
CAGTGGT806900.0137.991154
GCAGTGG813300.0136.800383
AGTGGTA816900.0136.293125
CAACGCA796600.028.60762810-14
TCAACGC796850.028.5823910-14
ATCAACG799150.028.47850410-14
GTACATG793400.028.45521220-24
AGAGTAC797000.028.44692615-19
TACATGG793450.028.42619520-24
CAGAGTA798250.028.40779315-19
CGCAGAG800350.028.4016215-19
AGTACAT797150.028.33038120-24
GCAGAGT801550.028.26209315-19
ACATGGG804050.027.89384320-24
Produced by FastQC (version 0.11.5)