FastQCFastQC Report
Sun 19 Mar 2023
SRR4895907_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895907_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81658
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG900.0142.541965
GATACTG100.0074502723142.541959
AGATACT100.0074502723142.541958
ACTTGCG100.0074502723142.541958
CATGATT100.0074502723142.541959
ATAGCTA202.3069697E-6142.541956
ATAGCAT202.3069697E-6142.541956
TACGGTG100.0074502723142.541957
TAAGTAG100.0074502723142.541957
TATGCGG100.0074502723142.541957
ATACTAG202.3069697E-6142.541956
TACATGA100.0074502723142.541957
TACATCG100.0074502723142.541957
CATAGTT2450.0142.541955
CATAGAT2050.0142.541955
ATGAGAC100.0074502723142.541959
ACATGAT100.0074502723142.541958
TAGATGA100.0074502723142.541957
ACATCGC100.0074502723142.541958
ATACGTC254.0761734E-8142.541956
Produced by FastQC (version 0.11.5)