FastQCFastQC Report
Sun 19 Mar 2023
SRR4895907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81658
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC42225.170344607999216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT13711.6789536848808444No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA12801.5675132871243478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA11261.3789218447671998No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA9501.163388767787602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAATCAACCGAGATTCCCC8120.9943912415195082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC6750.8266183350069802No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGAAATCAACCGAGAT6570.8045751794067942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTAATGAGGCGAACC6400.7837566435621739No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGCAGCCC6150.7531411496730266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAACCGAGATTCCCCCAGTA6150.7531411496730266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGAGATTCCCCCAGTAG5120.6270053148497392No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC4560.5584266085380489No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGATTCCCCCAGTAGCGG4250.5204633961155061No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACCGGGGGAACTGAAACA3980.48739866271522697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTAAGTACCCCGAGGAAA3790.4641308873594749No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATCTAAGTACCCCGAGGAA3630.44453697127042047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGACTAAGCGTACACGGT3520.4310661539591957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA3360.41147223787014137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3190.3906537020255211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCATCTCCTCTAAC2790.3416689118028852No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGAACGGGGAGCAGCCCA2690.32942271424722624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCAGAGCCTGAATCAGT2340.28656102280241985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2290.28043792402459033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTGTGAGGTTAAGCGACTA2260.27676406475789267No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGAGATTCCCCCAGTAGCG2230.273090205491195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAGTACCCCGAGGAAAAG2210.2706409659800632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTAATGAGGCGAACCGG2100.2571701486688383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATAGGTTAATGAGGCGAAC2000.24492395111317938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGTCGGTAAGGTGATATG1980.24247471160204753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGACTAAGCGTACACGG1950.23880085233534987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTC1850.22655465477969092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCTCCTCTAACT1780.2179823164907296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGAAGGACGTGCTAATCT1770.2167576967351637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1770.2167576967351637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATTCCCCCAGTAGCGGCG1690.20696073869063658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACTAAGCGTACACGGTG1680.20573611893507068No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATCAACCGAGATTCCCCC1650.202062259668373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAATTCTGGACATTAAT1640.20083763991280706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGTACACGGTGGATGCC1630.19961302015724117No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGGCAGTCAGAGGCGATGA1590.19471454113497758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGGACGTGCTAATCTGCG1540.18859144235714811No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCT1520.18614220284601632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.16532366700139609No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGAGCGAACGGGGAGC1350.16532366700139609No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCGTACACGGTGGATGC1310.16042518797913247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC1290.1579759484680007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTAAGCGACTAAGCGTA1280.1567513287124348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTCCCCCAGTAGCGGCGA1280.1567513287124348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAGCGTACACGGTGGATG1270.1555267089568689No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGGGGGAACTGAAACAT1230.1506282299346053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACGGGGAGCAGCCCAGAG1220.14940361017903941No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1150.14083127189007813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGTACCCCGAGGAAAAGA1130.13838203237894633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACTAAGCGTACACGGTGG1070.13103431384555095No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTAAGCGTACACGGTGGA1030.12613583482328738No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG1020.12491121506772147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGCGGCGAGCGAACGGGG1020.12491121506772147No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACA1020.12491121506772147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAATCAACCGAGATTCCC1010.12368659531215559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAACCGAGATTCCCCCAG980.12001273604545788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAACATCTAAGTACCCCGA960.11756349653432609No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGGTTAATGAGGCGAACCG910.11144039775649661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACGTGCTAATCTGCGATA870.10654191873423302No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA860.10531729897866712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCATAGGTTAATGAGGCGA860.10531729897866712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAAGGACGTGCTAATCTGC850.10409267922310123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT41350.0141.562271
AGTGGTA42250.0138.376345
CAGTGGT42300.0138.212774
AGCAGTG42400.0138.056612
GCAGTGG42600.0137.408453
GGTATCA42550.0137.40078
GTATCAA42550.0137.40079
GTGGTAT43900.0133.01146
TGGTATC45250.0129.043097
AGGTATC2850.028.80000180-84
CGCGGTT353.9759743E-628.800001135-139
GCGGTTA353.9759743E-628.800001135-139
CGGTTAC403.4490768E-728.800001135-139
TACGGTG200.006279031728.800001110-114
GGCGACT200.006279031728.80000125-29
GCACTTG255.361362E-428.80000175-79
CGGAGCT502.6248017E-928.80000175-79
CCGCAGA403.4490768E-728.80000190-94
TCGCGGT353.9759743E-628.800001135-139
CTTACGA200.006279031728.800001110-114
Produced by FastQC (version 0.11.5)