FastQCFastQC Report
Sun 19 Mar 2023
SRR4895906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011193
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG392521.9516774372225838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC241391.2002328965942106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA98160.4880685245026211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG83830.41681728208083463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83060.41298870869180626No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG56740.2821211092122934No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG56390.2803808485809169No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT47050.2339407505893268No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG43220.2148973271088354No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT42530.21146652757840745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC42180.20972626694703095No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT41540.20654407607822817No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT41490.20629546741660298No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT40640.20206912016897433No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC40090.19933442489109698No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG39440.19610251228996922No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA38840.19311920835046661No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA38120.1895392436230635No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT37700.1874509308654117No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT37450.18620788755728565No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT37130.18461679212288426No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT36060.1792965667641047No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC36050.17924684503177965No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG35700.17750658440040315No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC35540.17671103668320245No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT35480.1764127062892522No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT35400.17601493243065186No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA35350.17576632376902662No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC34960.17382717620834995No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT34920.1736282892790498No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC34690.1724846894355738No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT34380.1709433157334975No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT34170.1698991593546716No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG34120.16965055069304635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGTTACTGAGAACTCAT34010.1691036116374709No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA33970.16890472470817072No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC33800.168059455258645No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT33530.16671696848586884No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG33370.16592142076866814No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC33230.16522531651611755No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT32790.16303756029381566No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG32520.1616950735210395No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT32440.16129729966243916No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT32370.16094924753616385No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA31920.15871176958153693No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA31860.15841343918758666No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA31800.1581151087936364No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT31280.15552957871273418No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT31250.15538041351575907No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT31210.1551815265864589No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC31130.15478375272785855No Hit
AGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTA30960.15393848327833282No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT30330.1508060141418551No Hit
ATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTG29880.14856853618722818No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC29570.14702716248515185No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG29270.14553551051540056No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG29250.1454360670507505No Hit
TGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACT29190.14513773665680021No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG29180.14508801492447518No Hit
GTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGA28970.14404385854564927No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT28870.14354664122239885No Hit
AATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTT28620.14230359791427277No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA28430.14135888500009694No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT28240.14041417208592114No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA28140.13991695476267071No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA28140.13991695476267071No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC28100.13971806783337054No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC27980.13912140704547002No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC27960.13902196358081992No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA27950.1389722418484949No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA27950.1389722418484949No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG27730.13787836373734397No Hit
TATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGAT27380.13613810310596747No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG27140.13494478153016642No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT26880.1336520164897153No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT26770.13310507743413982No Hit
TTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGC26700.13275702530786454No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA26470.13161342546438853No Hit
AATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGG26430.1314145385350884No Hit
TACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGAT26370.1311162081411381No Hit
AATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAA26320.1308675994795129No Hit
GTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTT26300.13076815601486283No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA25960.12907761711581137No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC25740.12798373900466042No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC25720.12788429554001032No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG25700.12778485207536025No Hit
CGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAA25660.12758596514606008No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG25660.12758596514606008No Hit
ATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAA25620.1273870782167599No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT25460.1265915304995592No Hit
GTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCAC25440.12649208703490913No Hit
ATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTG25390.12624347837328392No Hit
TAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGG25390.12624347837328392No Hit
TGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAA25330.12594514797933365No Hit
ACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTT25290.1257462610500335No Hit
ATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGA25040.12450321774190741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT25030.12445349600958236No Hit
ACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTG24970.12415516561563211No Hit
TGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTT24640.12251434844890571No Hit
AGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAAT24630.12246462671658066No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC24570.12216629632263039No Hit
GCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTG24510.12186796592868014No Hit
AGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTAC24470.12166907899937997No Hit
TCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAA24470.12166907899937997No Hit
CAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACA24460.12161935726705492No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA24420.12142047033775476No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA24380.12122158340845458No Hit
GATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAG23610.11739301001942627No Hit
TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTT23250.11560302765572474No Hit
GGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTT22870.1137136018273731No Hit
GTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACT22820.1134649931657479No Hit
TTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTA22730.11301749757482252No Hit
CCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGC22690.11281861064552233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22620.11247055851924703No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC22520.11197334119599661No Hit
TTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTAC22420.11147612387274618No Hit
GACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCT22370.11122751521112097No Hit
GGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTT22320.11097890654949574No Hit
TTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGAC22140.11008391536764497No Hit
AGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCA22020.10948725457974445No Hit
TTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTG22000.10938781111509437No Hit
GTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAA21810.10844309820091855No Hit
AGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAATGG21700.10789615914534309No Hit
CTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTT21670.10774699394836797No Hit
CTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTG21570.10724977662511753No Hit
CTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGT21290.10585756812001633No Hit
TGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGC21100.10491285520584051No Hit
GCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCT21030.10456480307956523No Hit
ACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTT20930.10406758575631479No Hit
TTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCT20860.10371953363003947No Hit
AACAGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTG20740.10312287284213897No Hit
AACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACT20640.10262565551888854No Hit
GAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGG20550.10217815992796316No Hit
TAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATAT20440.10163122087238768No Hit
GATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGAT20350.1011837252814623No Hit
ATATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGG20250.10068650795821187No Hit
CTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTT20200.10043789929658664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA346350.0139.776328
AAGCAGT347200.0139.451521
GTGGTAT348150.0139.01236
GTATCAA350150.0138.321099
TGGTATC350450.0138.12057
AGCAGTG354400.0136.557462
CAGTGGT355900.0136.06614
GCAGTGG356150.0135.846023
AGTGGTA369750.0130.910965
CAACGCA346100.028.62450210-14
ATCAACG346100.028.60369910-14
TCAACGC347850.028.48463410-14
TACATGG345650.028.44930320-24
AGAGTAC348450.028.33640715-19
GTACATG348400.028.2288820-24
CAGAGTA351000.028.13874815-19
AGTACAT351100.028.03640620-24
CGCAGAG353350.027.98828315-19
GCAGAGT352650.027.95400815-19
ACATGGG353950.027.7740420-24
Produced by FastQC (version 0.11.5)