FastQCFastQC Report
Sun 19 Mar 2023
SRR4895905_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895905_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824604
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC825050.0142.455432
ATAGCGA825500.0142.377781
AGCGACT247150.0142.042953
AGCGACG252600.0141.912483
GCGACAT69400.0141.711644
AGCGACA206250.0141.4623
AGCGACC123250.0141.203253
GCGACGA66250.0141.134724
GCGACTA65900.0141.019154
GCGACGT82900.0140.64194
GCGACTG79900.0140.30334
GCGACTT79050.0139.91874
GCGACAG63850.0139.742784
GCGACGG83250.0139.365774
CGACGTA23500.0139.197285
GCGACTC25450.0139.172874
CGACATA19750.0138.924735
CGACTGA21650.0138.912235
GCGACAA59050.0138.06774
CGACGAT23100.0137.905475
Produced by FastQC (version 0.11.5)