FastQCFastQC Report
Sun 19 Mar 2023
SRR4895904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347074
Sequences flagged as poor quality0
Sequence length150
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA114613.3021776335882262No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53311.5359836807136231No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC45361.3069259005284175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA38951.1222390614105349No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC38461.1081210347072958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA37951.0934267620161693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT33140.9548396019292715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAAAAAAAAAAAAAA24980.7197312388712493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA22950.6612422711006875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA22040.6350230786518148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20110.5794153408206895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT19740.5687547900447744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18700.5387899986746343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT17940.5168926511349164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCAATAGATATAGTACCG16470.4745385710251992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA15970.46013242132801646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA11230.3235621221987242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAAAAAAAAAAAAAAAAA9940.28639425597999274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAAAAAAAAAAAAAAA9180.264496908440275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACCCGTCTATGTA9050.2607513095190075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTACAGCAAAAAAAAAAAA8800.2535482346704161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTTAATTTATTAATG8680.2500907587430923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA8200.23626085503379685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACACCCGTCTATGTAG7610.2192615983911212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACAGCTAAAAGAGCACAC7560.21782098342140294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA7130.2054316946818258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAAAAAAAAAAAAAAAAA7020.20226234174844557No Hit
AAGCAGTGGTATCAACGCAGAGAACATGGGGAAAAAAAAAAAAAAAAAAA6900.19880486582112172No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA6690.19275428294830496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAAAAAAAAAAAAAAAAA6430.18526308510576994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA6370.18353434714210803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAACCAAGCATAATATAGC6080.17517878031774203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCAAGCATAATATAGCAA6040.17402628834196743No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACAAACCTACCGAGCCT5590.16106075361450298No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA5580.1607726306205593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA5570.16048450762661565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAAAAAAAAAAAAAAAAA5560.160196384632672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAAAAAAAAAAAAAAAA5390.15529829373562987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAATGAATTAACTAGAAAT5380.15501017074168622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA5310.15299330978408063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCACAAGTTCAAAAAAAAAA5090.14665460391732021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA4970.14319712798999637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAAAAAAAAAAAAAAAA4890.14089214403844713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC4850.1397396520626725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAAAAAAAAAAAAAAAAA4800.13829903709295424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAAAAAAAAAAAAAAAAA4570.13167220823225018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAACCAAGCATAATATAGCA4470.12879097829281363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4400.12677411733520805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTTCAAAAAAAAAAAAAA4360.12562162535943344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAACTTTGCAAGGAGAGCCA3970.1143848285956309No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAAAAAAAAAAAAAAAAA3900.11236796763802533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA3830.11035110668041972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTCCAAAGAGGAACAGCTC3800.10948673769858878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT100.007240151143.979549
AAGCAGT255250.0143.533921
GTGGTAT261100.0140.31256
AGTGGTA261200.0140.231225
GCAGTGG261050.0140.206943
GGTATCA261350.0140.205838
GTATCAA261600.0140.181939
CAGTGGT261350.0140.178284
TGGTATC262100.0139.804637
AGCAGTG262050.0139.616932
TTTGAGT203.9739994E-4107.984654
AGTGTTA301.6550142E-595.986365
CGTGGTA259.6484734E-486.3877264
GCAGTGT451.2806959E-680.0232243
TCAGTGG451.2806959E-680.0232243
TGTTATC451.2351845E-463.9909067
AGCAGTT607.0687456E-660.0174182
TGTATCA607.086892E-659.9914748
GTTGTAT607.086892E-659.9914746
ATGCAGT502.0753115E-457.616721
Produced by FastQC (version 0.11.5)