FastQCFastQC Report
Sun 19 Mar 2023
SRR4895903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391565
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATG2394400.0142.36371
GCCATGC2396000.0142.268632
CCATGCG752050.0141.898443
CCATGCA567650.0141.616853
CATGCGT243700.0141.334354
CCATGCT693950.0141.280443
CATGCGA185600.0140.728654
CCATGCC396900.0140.414573
CATGCAG189300.0140.312154
CATGCGG253600.0140.25714
CATGCAT188600.0139.812684
CATGCAA152250.0138.60144
CATGCTG231800.0138.505784
ATGCGAT65800.0138.417655
CATGCTT218600.0138.066974
ATGCGTA69750.0137.833335
CATGCGC76800.0137.800814
ATGCGAG60050.0137.430165
CATGCCG106900.0137.266684
ATGCGAA52000.0135.40675
Produced by FastQC (version 0.11.5)