FastQCFastQC Report
Sun 19 Mar 2023
SRR4895903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391565
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG236520.9889758379972947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC150040.6273716164938022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA96560.40375235462970904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91330.3818838292080709No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG71920.30072358476562416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC63840.26693817646603796No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG45690.19104644866436832No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC45340.189582971819708No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG42910.17942226115535226No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCTCAATCCGCAAAAAAAAAAAAA39310.16436935646741777Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT37460.15663383600278477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA34730.14521871661443447No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT34430.14396430789043996No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT34060.14241720379751335No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG33800.1413300495700514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGTTACTGAGAACTCAT32930.13769226427046724No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG32540.13606153292927434No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT32480.13581065118447544No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT32260.1348907514535461No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC31870.13326002011235322No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA30660.12820057159224188No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT30400.12711341736477996No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT29820.1246882271650572No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT29220.12217940971706812No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC29170.12197034159640235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC29100.1216776462274703No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC28860.12067411924827466No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA28820.12050686475174206No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT28650.11979603314147848No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC28470.11904338790708177No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG28320.1184161835450845No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA28230.11803986092788614No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT28160.11774716555895408No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT27740.11599099334536171No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT27650.11561467072816335No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC27600.1154056026074976No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT27530.11511290723856554No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC27160.11356580314563895No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG26920.1125622761664433No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT26880.11239502166991071No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG26690.11160056281138084No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT26550.1110151720735167No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC26200.10955169522885641No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG26150.10934262710819068No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT25830.1080045911359298No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT25650.1072519459015331No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA25600.10704287778086734No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA25510.10666655516366898No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA25340.1059557235534054No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT25180.10528670556727499No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT25100.10495219657420977No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT24970.1044086194604788No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT24710.10332146523301688No Hit
AGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTA24530.10256881999862015No Hit
ATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTG24380.10194161563662288No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT24010.10039451154369627No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT23960.10018544342303053No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA23950.10014362979889739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT542900.0140.327011
GGTATCA545400.0139.637058
GTGGTAT545700.0139.494326
TGGTATC548250.0138.976847
GTATCAA549000.0138.773869
CAGTGGT555300.0137.199454
AGCAGTG555600.0137.002722
GCAGTGG560050.0135.901283
AGTGGTA566450.0134.460685
GACTGGA18750.055.6960333
GTGACTG18950.054.7281571
AGTTCAG20700.051.128059
TGACTGG20500.050.2388572
ACTGGAG21650.047.8869134
TGGAGTT21600.047.6644446
CTGGAGT24150.042.9296765
GAGTTCA28350.037.3315938
GGAGTTC29600.034.782167
CAACGCA545150.028.62432710-14
ATCAACG544800.028.6004310-14
Produced by FastQC (version 0.11.5)