FastQCFastQC Report
Sun 19 Mar 2023
SRR4895901_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895901_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201398
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGA100.007459282142.535897
CTATAGC100.007459282142.535898
TACGGGC1250.0142.535896
ACGATTC151.3153667E-4142.535897
TACGACC202.3157845E-6142.535896
GTACTAA4300.0142.535895
ACTCGTA100.007459282142.535897
CGATCGA100.007459282142.535898
CGTCATA151.3153667E-4142.535898
CGATATC100.007459282142.535898
TACCGTC700.0142.535896
ACGGTCG151.3153667E-4142.535897
GATGTAC201550.0142.394442
ATGTACG64550.0142.315063
AGATGTA201800.0142.218031
GTACTGG7050.0141.5255
ATGTACA47450.0141.33433
TGTACTA15650.0141.169744
ATGTACT61300.0141.140753
TGTACGA14650.0141.076484
Produced by FastQC (version 0.11.5)