FastQCFastQC Report
Sun 19 Mar 2023
SRR4895901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201398
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG25221.2522467949036238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT22401.1122255434512756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18830.9349645974637286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC17240.8560164450491067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC6680.3316815459935054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5370.26663621287202455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC5210.25869174470451545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG4030.20010129196913573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTC2560.12711149068014577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAATTCTGGACATTAAT2490.12363578585686055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA2200.10923643730325029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT42000.0139.885711
GGTATCA42400.0138.735868
GTGGTAT42300.0138.72346
CAGTGGT42700.0137.255284
AGCAGTG43000.0136.632552
GTATCAA43100.0136.48269
TGGTATC43150.0136.324457
GCAGTGG43450.0135.051793
AGTGGTA44500.0132.026985
ACACCCG350.0036613861.7142837
CACCCGT450.00989488648.0000048
TAACGGT353.9835995E-628.80000185-89
GTTCGAT353.9835995E-628.800001130-134
CGTACGG502.6357156E-928.80000150-54
TCGTACG502.6357156E-928.80000150-54
TCGCGGT502.6357156E-928.800001135-139
ACCGTAA453.013156E-828.8140-144
CAACGCA42250.028.62958710-14
ATCAACG42350.028.52798310-14
GTACATG42100.028.45795820-24
Produced by FastQC (version 0.11.5)