FastQCFastQC Report
Sun 19 Mar 2023
SRR4895900_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895900_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928436
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC930200.0142.274032
ACAGATT931150.0142.128881
AGATTCG281300.0141.96713
GATTCGA73150.0140.990884
AGATTCA229550.0140.748983
GATTCGG87200.0140.588184
AGATTCT301200.0140.58583
GATTCGT96900.0140.416784
GATTCGC26800.0140.156314
GATTCAG74300.0139.000524
GATTCCG33600.0138.94374
GATTCTG95900.0138.90814
GATTCTA81100.0138.419274
ATTCGAT25800.0137.57725
AGATTCC129300.0137.313133
ATTCTAG24900.0136.824975
ATTCGGA22300.0135.518265
ATTCGTA27850.0135.383995
ATTCGAA20650.0135.301565
ATTCGTG29450.0135.047245
Produced by FastQC (version 0.11.5)