FastQCFastQC Report
Sun 19 Mar 2023
SRR4895899_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895899_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104574
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC1108150.0142.09051
CACGACC1108700.0142.020022
ACGACCT325700.0142.006533
ACGACCG344200.0141.746023
CGACCTA91150.0141.615254
ACGACCA269650.0141.416993
CGACCGT111350.0140.76134
GACCGTA29400.0140.61415
CGACCGA87350.0140.513644
CGACCAT89350.0140.319994
CGACCTG104050.0140.019034
ACGACCC173800.0139.723883
CGACCAG87650.0139.463474
CGACCTT107150.0139.427154
CGACCGG116550.0139.251224
GACCGAT29700.0137.993845
CGACCAA76100.0137.776864
GACCTAG28600.0137.070795
GACCGGT39600.0136.793875
GACCGTG36700.0136.727175
Produced by FastQC (version 0.11.5)