FastQCFastQC Report
Sun 19 Mar 2023
SRR4895899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104574
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG171651.553992761010127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC129611.1733935435742648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37890.343028171946832No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT22470.2034268414791585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA20070.1816990079433338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19560.17708184331697108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC17310.15671199937713542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC14710.1331735130466587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG13700.12402971643366582No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT12230.11072141839297321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC11370.10293561137596939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT219350.0139.52291
GGTATCA220050.0139.152048
GTGGTAT221000.0138.521296
GTATCAA221700.0138.213849
TGGTATC223550.0137.005627
CAGTGGT224800.0136.211784
AGCAGTG224650.0136.070972
GCAGTGG226350.0135.303513
AGTGGTA238350.0128.468255
TCGTACG850.028.79913950-54
CAACGCA219550.028.53653310-14
ATCAACG219350.028.5297310-14
TCAACGC221100.028.32996710-14
TACATGG219950.028.28245720-24
AGAGTAC221050.028.1995815-19
GTACATG220450.028.19871320-24
AGTACAT220850.028.16068320-24
CAGAGTA222050.028.07258415-19
CGCAGAG222500.028.04169315-19
GCAGAGT222750.027.94557815-19
Produced by FastQC (version 0.11.5)