FastQCFastQC Report
Sun 19 Mar 2023
SRR4895898_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895898_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157379
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC2164100.0142.091222
AATGTTG2174250.0141.42791
TGTTGCA502100.0140.63383
TGTTGCG648400.0140.216613
GTTGCGA157000.0140.039154
GTTGCTA172300.0139.847234
TGTTGCT709450.0138.62813
GTTGCAT169900.0138.4254
GTTGCAG163500.0138.264084
GTTGCGT219050.0137.948364
GTTGCAA138200.0136.863244
GTTGCGG220800.0136.75824
GTTGCTG235000.0135.803254
TGTTGCC351350.0135.537813
TTGCGAT60300.0135.444475
TTGCGTA61200.0134.966495
TTGCTAG59950.0133.382115
TTGCGAG51750.0132.482545
GTTGCGC64650.0131.732624
TGCGATA16700.0131.44026
Produced by FastQC (version 0.11.5)