FastQCFastQC Report
Sun 19 Mar 2023
SRR4895897_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895897_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620548
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC621050.0142.419772
AATCCGT621850.0142.236541
TCCGTCG188850.0141.413793
TCCGTCT190350.0140.561543
CCGTCTA49700.0140.394914
CCGTCGA47500.0140.145254
CCGTCGT70500.0140.01864
TCCGTCA154800.0139.13893
TCCGTCC96700.0139.081863
CCGTCAA41400.0138.930714
CCGTCTG61000.0138.923954
CCGTCAG45800.0138.655564
CGTCGAT16300.0138.610695
CCGTCGG56950.0138.04064
CGTCGTA18650.0137.960085
CGTCAAT14950.0137.77865
CGTCGGT20300.0136.928445
CGTCGAG14700.0136.727815
CGTCGAA14000.0136.43695
CGTCTAT17450.0136.010965
Produced by FastQC (version 0.11.5)