FastQCFastQC Report
Sun 19 Mar 2023
SRR4895896_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895896_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285273
Sequences flagged as poor quality0
Sequence length150
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTA151.3163057E-4142.525868
CGGATAG600.0142.525868
CGTAGCA151.3163057E-4142.525868
ACGCTGT151.3163057E-4142.525867
CGATATA307.3123374E-10142.525868
AGCGTAG100.0074624703142.525859
ACTGAAC100.0074624703142.525857
ATCAGTA100.0074624703142.525859
ACCGATA254.105459E-8142.525857
CGTCTAG100.0074624703142.525858
GAATAAG100.0074624703142.525859
CATTCGA100.0074624703142.525858
CTGACGA100.0074624703142.525858
CCATGAC100.0074624703142.525858
ACAGGTA700.0142.525857
CTAGTAA351.2732926E-11142.525858
CAGATAG202.318271E-6142.525858
CAGATAA202.318271E-6142.525858
ACTAATG900.0142.525857
ACGTCTA202.318271E-6142.525857
Produced by FastQC (version 0.11.5)