FastQCFastQC Report
Sun 19 Mar 2023
SRR4895896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285273
Sequences flagged as poor quality0
Sequence length150
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA93253.268798659529643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAATCCGCAAAAAAAAAAAA66912.3454725824035223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCCGCAAAAAAAAAAAAA52461.838940243205631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37081.299807552765246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA34651.2146259898413099No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA28280.9913311109007863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT24820.8700437826222601No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGCAAAAAAAAAAAAAAAA24260.8504134635945216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA23450.8220196092865432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22680.7950279206234029No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCAATCCGCAAAAAAAAAA21750.7624275693809088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA21410.750509161399782No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAATAAAGCCTTTTAAAT20500.7186098929797072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACACCCCGATCCTCCCAC17760.6225615463082731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATCATCAAAAAAAAAAAA16200.5678770861595734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA14770.5177496643565987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTTAAAAAAATCTAAAT12710.4455381336474184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAAAAAAAAAAAAAAAAA10020.35124249403203245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTCAAAAAAAAAAAAAAA9140.3203948498455865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCATTTAAAAAAAAAAAAA8770.3074248176308308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCGCAAAAAAAAAAAAAA8220.2881450400143021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATCATCATTCAAAAAAAA7790.2730717593322887No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC7620.2671125553417253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA7550.264658765463258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCCTGAAAAAAAAAAAA7470.2618544341735811No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTTGTTCCACTGCAAAT7390.25905010288390423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTCCACTGCAAATAAAGCC7290.2555446887718081No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTGCACAAGTTCAAAAAA7260.2544930645381792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCACAAGTTCAAAAAAAAAA7050.24713169490277734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCAAAAAAAAAAAAAAAA6660.23346057986560242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC6620.23205841422076398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGACCCCCGCTTCCAGGA6580.2306562485759255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCCTCGGACACCCCGA6490.227501375875039No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA6450.22609921023020052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAAAAAAAAAAAAAA6350.2225937961181044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCAAAAAAAAAAAAAAAAA5960.2089226810809295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCATTCAAAAAAAAAAAAA5910.20716997402488144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACGATTCGCAAAAAAAAAAA5490.19244723475407768No Hit
AAGCAGTGGTATCAACGCAGAGAACATGGGGAAAAAAAAAAAAAAAAAAA5470.19174615193165845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCCTGCAAAAAAAAAAA5310.18613748935230465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTTGAAAAAAAAAAAAAA5300.18578694794109502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGACACCCCGATCCTCCCA4920.17246637431512973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACTTGAAAAAAAAAAAA4840.16966204302545282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAAAAAAAAAAAAAAAAA4810.16861041879182398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGGGGGTCCCAAGGA4580.16054796633400287No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTCGCAAAAAAAAAAAAA4480.15704255222190674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA4370.153186596698601No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTTTGCCCCCTGCAAAAA3840.1346079019044915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT3790.13285519484844344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTTCAAAAAAAAAAAAAA3740.13110248779239536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGAAACCCCGTCTCTACT3690.1293497807363473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTACAGTACAATCAGCA3680.12899923932513768No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA3670.12864869791392805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAAAAAAAAAAAAAAAA3640.12759707368029924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGATTTACTGAAAAAAAAA3470.12163786968973579No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCATTTCAAAAAAAAAAAA3460.12128732827852617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTAATAAAGTTATCTGC3400.11918407981126851No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGGCTTCTCAATCCGCAAA3270.11462704146554352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC3240.1135754172319147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA3220.11287433440949547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA3200.11217325158707624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTACAGCAAAAAAAAAAAA3180.111472168764657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACACCCCGATCCTCCCACA3170.11112162735344741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTGCACAAGTTCAAAAAA3130.10971946170860894No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAATTCTGGACATTAAT3100.1086678374749801No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGGCAAAAAAAAAAAA3090.10831729606377048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCGCAAAAAAAAAAAAAAA3080.10796675465256086No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTC3050.10691513041893204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAATAAAGGCTTCTCAATC3020.10586350618530321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAATCCGCAAAAAAAAAAA2950.1034097163068359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTTACTGAAAAAAAAA2950.1034097163068359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACCCGTCTATGTA2890.10130646783957822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA2880.10095592642836862No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTGTTCCACTGCAAATA2860.10025484360594938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT219700.0143.802141
AGCAGTG223650.0141.069212
AGTGGTA224750.0140.699145
CAGTGGT225000.0140.63884
GCAGTGG224800.0140.571763
GTGGTAT225100.0140.512346
GTATCAA226000.0140.17589
GGTATCA226100.0139.986428
TGGTATC226400.0139.769137
ACGCAGT259.6415804E-486.399261
AGGGTAT259.6415804E-486.399265
CGCAGTG259.6415804E-486.399262
TGGTGTC259.6415804E-486.399267
ACTGGAG353.5530873E-582.285014
AGCAGTT554.598769E-878.5447852
GTGACTG406.887439E-571.999391
GACTGGA406.887439E-571.999393
AGTGTTA300.001988223871.999395
TGACTGG451.2339931E-463.999462
TCAGTGG808.51287E-962.9994623
Produced by FastQC (version 0.11.5)