FastQCFastQC Report
Sun 19 Mar 2023
SRR4895895_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895895_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491339
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC492350.0142.222092
AAGAGAT493450.0141.905041
GAGATCG141950.0141.57223
GAGATCA118150.0141.500673
AGATCGA38900.0141.426884
GAGATCT172600.0141.246123
AGATCTA44500.0140.924644
AGATCAG36550.0140.771294
AGATCGT51100.0140.434194
GAGATCC62900.0140.373443
AGATCTG50250.0139.831534
GATCGAG13050.0139.795675
GATCGAT14000.0139.471925
GATCGTA14250.0139.025425
AGATCGG41800.0138.775384
AGATCAT41500.0138.576544
GATCGAA10200.0138.334125
GATCATG12900.0138.106645
GATCGTG15250.0137.853075
AGATCCG15200.0137.837694
Produced by FastQC (version 0.11.5)