FastQCFastQC Report
Sun 19 Mar 2023
SRR4895895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491339
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG82951.6882437583827052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC58051.1814653426656545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21830.4442960969920971No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21750.44266789324682143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13930.28351097714612516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC7890.16058159437781247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT6690.1361585381986775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC6590.13412328351708291No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT6390.13005277415389377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC5760.1172306696598479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG4940.10054158127077231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT103750.0139.826251
GGTATCA104400.0139.091848
GTGGTAT105350.0137.837596
GTATCAA105450.0137.63869
CAGTGGT106250.0136.737784
TGGTATC106650.0136.224917
AGCAGTG107700.0134.697982
GCAGTGG109250.0132.786943
AGTGGTA110200.0131.771225
TCGTACG750.028.79818550-54
CAACGCA104150.028.59022310-14
ATCAACG104250.028.53517310-14
AGAGTAC104150.028.43814715-19
TACATGG104150.028.3137220-24
GTACATG104700.028.20624520-24
CGCAGAG105350.028.19622415-19
CAGAGTA105050.028.180815-19
AGTACAT105100.028.11259520-24
TCAACGC106650.027.96053710-14
GCAGAGT105900.027.94101315-19
Produced by FastQC (version 0.11.5)