FastQCFastQC Report
Sun 19 Mar 2023
SRR4895894_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895894_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696350
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC1701850.0142.122341
ACTCACC1702500.0142.068072
CTCACCG548300.0141.633973
CTCACCT517350.0141.08113
TCACCGG180750.0140.808244
TCACCGA137150.0140.451924
TCACCGT182950.0140.439914
CTCACCA398850.0140.420353
TCACCTG166900.0140.062944
TCACCTA146600.0139.859854
TCACCAG128800.0139.206764
CACCGAT49800.0137.429535
TCACCAT134750.0137.080864
CACCGTA52750.0136.77175
CACCGGT61800.0136.699865
TCACCTT170500.0136.645664
ACCGGTA18150.0135.120126
CACCGTT65450.0134.740525
CACCGTG57850.0134.696095
CACCTGG58000.0134.593555
Produced by FastQC (version 0.11.5)