FastQCFastQC Report
Sun 19 Mar 2023
SRR4895893_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895893_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831787
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1833800.0142.380252
TTCACGC1835150.0142.27551
CACGCAT591100.0141.719713
CACGCAG563950.0141.440123
ACGCATA160050.0141.292764
CACGCAA458050.0141.04593
ACGCATG190200.0141.040764
ACGCAAT152100.0140.805884
ACGCAGT179950.0140.796954
ACGCATT175500.0140.671554
CGCATAG52400.0139.547355
ACGCAGG187050.0139.415244
ACGCATC69400.0138.94534
CACGCAC237200.0138.934053
ACGCAGA149800.0138.781054
CGCAAGT49650.0138.520365
CGCATGA49250.0138.487725
CGCAGTT58550.0138.279225
CGCATAT51650.0138.124045
CGCAGTA53500.0138.010285
Produced by FastQC (version 0.11.5)