FastQCFastQC Report
Sun 19 Mar 2023
SRR4895893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831787
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG233531.274875299366138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC145900.7964899849163685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86370.4715067854504918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA52470.2864416004699237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC50540.2759054409710299No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG45820.2501382529737355No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA28080.15329293198390423No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG26240.14324809598495894No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT25920.14150116798514237No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG25050.13675170748564108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23850.13020072748632894No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT22260.12152067898724032No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA21870.11939161048746388No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT20650.1127314474881632No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT20210.11032942148841542No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG19460.10623505898884533No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT19450.10618046748885104No Hit
GTGAACCCCGTGGTCTCCTACGCCGTGGCTCTCAGCTGTCAATGTGCACT18980.10361466698912045No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC18790.10257742848922936No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT18760.10241365398924657No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG18750.10235906248925229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA18710.10214069648927522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT305750.0139.722871
GGTATCA307300.0139.241688
GTATCAA309050.0138.49989
GTGGTAT309100.0138.291066
TGGTATC310350.0137.850077
AGCAGTG313900.0136.072192
CAGTGGT314500.0136.008164
GCAGTGG318000.0134.385743
AGTGGTA323750.0132.05555
AGTTCAG11200.041.7850959
TGACTGG12900.038.5205232
GACTGGA13400.036.545753
GTGACTG13800.034.9645961
CTGGAGT14700.031.8362665
TGGAGTT15900.030.7919986
ACTGGAG16100.029.0678924
GAGTTCA16300.028.7112318
CAACGCA307100.028.6029510-14
ATCAACG307700.028.48165710-14
AGAGTAC307750.028.37455415-19
Produced by FastQC (version 0.11.5)