FastQCFastQC Report
Sun 19 Mar 2023
SRR4895892_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895892_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866381
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA4893650.0141.752942
TGGTAGC886900.0141.71244
GGTAGCC716100.0141.433415
GTGGTAG1571650.0141.165013
TGGTATC810850.0141.071464
TGGTAAC564350.0140.906344
TGGTACC392450.0140.89554
GGTATCC607100.0140.693245
GTAGCCT218950.0140.33466
GTAGCCG235700.0140.159276
GTATCCG208250.0140.118186
GTGGTAA1207250.0140.033143
GTGGTAC551000.0139.56013
GTGGTAT1618700.0139.54083
GGTAACC446450.0139.498935
GTAACCG152900.0139.005456
GGTACCC340600.0138.886125
TGGTGGT5001300.0138.701811
GGTATCG87300.0137.649615
GTATCCT193200.0137.235816
Produced by FastQC (version 0.11.5)