FastQCFastQC Report
Sun 19 Mar 2023
SRR4895891_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895891_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953434
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA1960350.0142.036422
TGGCTTC1961000.0141.989351
GCTTCAG617750.0141.976463
GCTTCAA496150.0141.536243
CTTCAGA163750.0139.843284
GCTTCAC224350.0139.777983
CTTCAGT202700.0139.623434
CTTCAGG202600.0139.094314
GCTTCAT641850.0138.899673
CTTCAAT170450.0138.736764
CTTCAAG159200.0138.467884
CTTCATG210200.0138.269594
CTTCACG59750.0135.265584
CTTCAGC61850.0134.590774
TTCAGTA61100.0133.326725
CTTCATA182550.0132.195684
TTCAGAG57500.0132.006095
CTTCACT61650.0129.709554
TTCAGGT70250.0129.25145
TTCAATG62200.0129.020945
Produced by FastQC (version 0.11.5)