FastQCFastQC Report
Sun 19 Mar 2023
SRR4895891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953434
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG161690.8277218477819062No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA121430.6216232542281951No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC97950.5014246706057128No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG94430.4834051214425468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC93940.4808967182919925No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61420.3144206561368339No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG58450.2992166615304126No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG57290.2932784010107329No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT48380.24766641719146898No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG45190.2313362007623498No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT44870.22969805992933467No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT44770.22918614091901748No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT43640.22340145610243292No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA43500.22268476948798885No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC41910.2145452572239451No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG41890.21444287342188167No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT41460.21224162167751767No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT40150.2055354826423621No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA39220.20077463584641203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGTTACTGAGAACTCAT38880.19903411121133346No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA38390.1965257080607791No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT38240.1957578295453033No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT38190.19550187004014466No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC37900.19401730491022476No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT37870.1938637292071296No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG37500.1919696288689559No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT37290.19089459894728975No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC36990.1893588419163381No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA36900.1888981148070526No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT36520.18695282256784718No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT36440.1865432873595934No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT36340.1860313683492762No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC36120.1849051465265783No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT36090.18475157082348315No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC36040.18449561131832456No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT35720.18285747048530945No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC35540.18193601626673847No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT34730.17778947228316902No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG34630.1772775532728518No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG34520.17671444236150288No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC34500.17661205855943943No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG33910.17359173639856787No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT33900.17354054449753614No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA33880.1734381606954727No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT33640.17220955507071137No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA33100.1694451924149984No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA32950.1686773138995226No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT32840.16811420298817364No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC32130.1644795780149214No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT31980.16371169949944558No Hit
ATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTG31760.16258547767674772No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT31680.16217594246849396No Hit
GTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGA31450.16099852874476436No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA31340.16043541783341542No Hit
AATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTT31200.1597187312189713No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA31170.15956515551587613No Hit
AGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTA30990.15864370129730515No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG30740.1573639037715121No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT30620.15674960095913146No Hit
TGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACT30310.15516265202714807No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC30150.15434358161064055No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT29610.15157921895492757No Hit
TATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGAT29260.14978750241881733No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA29140.14917319960643666No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC29120.14907081580437323No Hit
AATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGG29010.14850770489302428No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG28960.14825174538786567No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG28960.14825174538786567No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC28920.1480469777837388No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT28670.14676718025794575No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG28640.14661360455485056No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA28560.14620406934659683No Hit
ATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTG28450.14564095843524788No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA28430.14553857463318445No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG28340.14507784752389893No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT28250.14461712041461344No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT28150.14410520140429622No Hit
AATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAA28150.14410520140429622No Hit
ATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGA28040.14354209049294728No Hit
TTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGC27890.14277421197747148No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA27680.1416991820558053No Hit
AGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAAT27550.14103368734239294No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC27320.13985627361866335No Hit
GTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTT27170.13908839510318752No Hit
CAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACA27100.13873005179596548No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG26970.13806455708255308No Hit
ATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAA26860.13750144617120413No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC26840.1373990623691407No Hit
GCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTG26700.1366823757546966No Hit
ACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTG26510.1357097296350939No Hit
ACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTT26490.13560734583303044No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG26370.1349930430206498No Hit
TAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGG26290.134583507812396No Hit
GTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCAC26060.1334060940886664No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA26060.1334060940886664No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC26050.13335490218763468No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA26010.13315013458350783No Hit
TACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGAT25710.13161437755255617No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC25700.13156318565152444No Hit
TCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAA25650.13130722614636584No Hit
AGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTAC25560.13084649903708034No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT25240.12920835820406526No Hit
CGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAA25210.1290547825009701No Hit
GTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACT24970.12782617687620879No Hit
TGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAA24920.12757021737105015No Hit
GATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAG24780.12685353075660605No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA24680.12634161174628886No Hit
AGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCA24330.12454989521017859No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT24310.12444751140811515No Hit
GGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTT24290.1243451276060517No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC24110.12342367338748071No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC24070.12321890578335384No Hit
AGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAATGG23930.12250221916890972No Hit
CCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGC23870.1221950677627194No Hit
TGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTT23590.12076169453383119No Hit
TTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTG23570.12065931073176775No Hit
CTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTG23340.11948189700803816No Hit
TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTT23320.11937951320597472No Hit
CTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGT23310.11932832130494299No Hit
GGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTT23230.11891878609668921No Hit
TTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTA23190.11871401849256234No Hit
CTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTT23130.11840686708637199No Hit
GTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAA22980.11763898857089618No Hit
AACAGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTG22810.11676872625335691No Hit
TTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTAC22600.11569369633169074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22380.11456747450899288No Hit
ATATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGG22380.11456747450899288No Hit
TTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACC22330.11431151500383427No Hit
TTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGAC22230.11379959599351705No Hit
AACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACT22050.11287814177494607No Hit
GCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCT21890.11205907135843853No Hit
GTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACC21500.11006258721820139No Hit
TTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCT21420.10965305200994761No Hit
ATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCG21290.10898755729653524No Hit
GACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCT21180.10842444638518631No Hit
GATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGAT21170.10837325448415458No Hit
AGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAA21010.10755418406764702No Hit
CACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCA20930.10714464885939325No Hit
CACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTT20880.10688868935423465No Hit
ACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTT20790.10642796224494915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20770.1063255784428857No Hit
TTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTA20620.10555769992740989No Hit
GAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGG20600.10545531612534643No Hit
CTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTT20580.105352932323283No Hit
ACAGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAG20420.10453386190677545No Hit
TGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGC20380.10432909430264856No Hit
GTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATC20260.1037147914902679No Hit
TAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATAT20230.10356121578717274No Hit
TTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAA20200.10340764008407759No Hit
AACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAAT20030.1025373777665383No Hit
CTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGC19900.10187188305312593No Hit
GCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGT19900.10187188305312593No Hit
TACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCT19680.10074566123042805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT262400.0140.494551
GGTATCA263800.0139.685888
GTGGTAT263950.0139.524666
GTATCAA264700.0139.265359
TGGTATC265350.0138.842797
CAGTGGT268550.0137.188374
AGCAGTG269750.0136.532942
AGTGGTA270450.0136.197955
GCAGTGG272300.0135.254363
AGTTCAG10850.048.4417849
GACTGGA11200.046.296983
GGAGTTC13250.039.1240357
TGGAGTT13900.038.848436
GAGTTCA14300.036.2512938
CTGGAGT15150.034.2173925
GTGACTG17050.032.1016541
ACTGGAG16600.031.2285214
CAACGCA263050.028.58096310-14
ATCAACG262950.028.57540310-14
TCAACGC264500.028.42972410-14
Produced by FastQC (version 0.11.5)