FastQCFastQC Report
Sun 19 Mar 2023
SRR4895890_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895890_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84276
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC100.007448947142.553128
AATGCAT100.007448947142.553127
AGTTGCG100.007448947142.553129
ACAATGC750.0142.553125
ACAATAG2600.0142.553125
AGATTGA100.007448947142.553128
CAAGATG950.0142.553126
ATCGGCA100.007448947142.553128
CAATCGA100.007448947142.553126
CAATCAT254.0754458E-8142.553126
GAACAAT29600.0141.589943
TGGAACA86500.0138.84511
ACAATGA3750.0138.75175
AACAATG10950.0137.996634
AACAATA8100.0137.273364
AACAAGT9600.0136.61344
GGAACAA88350.0135.937742
GAACAAA21300.0135.191213
CAATATG950.0135.050316
GAACAAC8550.0134.216673
Produced by FastQC (version 0.11.5)