FastQCFastQC Report
Sun 19 Mar 2023
SRR4895889_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895889_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110442
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG3135800.0141.395451
GAAGAGA3147750.0140.858642
AAGAGAT1012450.0140.10023
AAGAGAG1011900.0137.260283
AAGAGAC380050.0136.323723
AAGAGAA841700.0131.685673
AGAGACC124650.0127.4545444
AGAGATG361200.0127.178214
AGAGATA309300.0126.834084
AGAGACG111050.0126.504374
AGAGAGT331350.0126.503264
AGAGATC135200.0125.4166644
AGAGACT97850.0124.3398064
AGAGATT322800.0123.053224
AGAGAAT301300.0122.631884
GAGATAT104900.0121.215145
GAGATAG108250.0120.426855
AGAGAGG379400.0119.8750844
GAGAGTC28950.0119.6534355
GAGATGA98150.0119.457425
Produced by FastQC (version 0.11.5)